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Monitoring of CAR-T lymphocytes in the course of hemato-oncological therapy
Dvořáková, Lenka ; Souček, Ondřej (advisor) ; Řezáčová, Vladimíra (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author Bc. Lenka Dvořáková Supervisor RNDr. Ondřej Souček, Ph.D. Title of Thesis Monitoring of CAR-T lymphocytes in the course of hemato-oncological therapy The diploma thesis deals with the therapy of T cells with a chimeric antigen receptor (CAR) in the treatment of blood malignancies. Specifically, it focuses on monitoring the population of CAR-T cells in patients over time, after the administration of a therapeutic product. Measurements were made from whole blood samples by measurement on a flow cytometer. The theoretical part is devoted to hematological malignancies: their diagnosis and treatment, as well as to CAR-T cells: history, production, clinical use, side effects and determinations. The experimental part is dedicated to processing of the measured results. The goal of the thesis was to prepare a research on the given topic and evaluate the results of monitoring the population of CAR-T cells over time. The performed technique was able to demonstrate CAR-T cells in the blood of all patients, flow cytometry using a test tube from the company Exbio and using the antiFMC63 scFv antibody from the company Acro Biosystems appears to be a suitable method. The rate of expansion of...
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